New Grad Student Opportunity: Free Cryo-EM Workshop

The Van Andel Research Institute is hosting a cryo-EM workshop August 13-15, 2018 in Grand Rapids, Michigan. Full scholarships, including travel, are available for up to 15 graduate students. Applications are due May 11, 2018.

Taught by internationally recognized cryo-EM experts, Cryo-EM at the Van Andel Research Institute will give gifted graduate students an intense overview of the latest innovative methodology and its applications across several fields including cancer and neurodegenerative disease research. By the end of the week, students will have a greater appreciation of what can be achieved through the application of cryo-EM.

Sessions will cover:

  • Electron microscopy
  • Electron tomography
  • Image processing basics
  • Image processing and 3-D reconstruction (hands-on session)
  • Negative stain and cryo-EM grid preparation
  • Cryo-EM on the Arctica and the Krios with a Volta phase plate

The course is designed to engage students and promote discussion between fellow participants and course instructors. In addition to talks and hands-on lessons, there will be social events that will provide opportunities to network.

For more information and to apply please visit the workshop website. Questions can be directed to Aubrie Bruinsma at aubrie.bruinsma@vai.org.

 

Raman Spectroscopy Workshop at Harvard

Horiba Scientific and Harvard’s Center for Nanoscale Systems will be hosting a two day Raman spectroscopy workshop May 15 – 16.  The lectures will be focused on Raman spectroscopy of 2D materials, but anyone with interest in learning the fundamentals and applications of Raman spectroscopy in greater detail should attend.

There will be a poster session and cocktail reception the evening of May 15th.  Please consider submitting a poster to help foster discussion about the topics that interest you and the challenges that you face in your research.  Poster abstracts should be sent to joanne.lowry@horiba.com.

David Tuschel from Horiba Scientific will be presenting the lectures at the workshop.  David has more than 30 years of experience in optical characterization of materials.  David shares responsibility with Fran Adar also from Horiba for authoring the Molecular Spectroscopy Workbench, which appears regularly in Spectroscopy magazine.

Since space in the workshop is limited there is a small ticket fee to discourage no shows.  Tickets are available here.

More information can be found in this flier.

How to Make It and Use It – A Microfluidic Workshop

The Center of BioModular Multi-Scale Systems in conjunction with RTNN will host a microfluidic workshop at the Chapel Hill Analytical and Nanofabrication Laboratory (CHANL). This workshop will offer participants the opportunity to learn about patterning structures across different length scales (nm→μm) and become familiar with a number of important application areas – microfluidics and nanofluidics for biological/biomedical applications. There will be lectures (mornings) covering relevant technological and application areas. In the afternoons, there will be on-site demonstrations of fabrication
equipment and hands-on experiments focused on making a microfluidic device and using it. More details can be found here.

Register here. RTNN is able to offset costs associated with registration and travel to a limited number of participants who demonstrate need. To apply for support, please visit the RTNN support page.

Atomic Force Microscopy (AFM) Workshop

Join us for presentations by academic and industry researchers conducting AFM work. There will also be demonstrations of Bruker’s Dimension Icon with time devoted to test samples. Coffee, tea and donuts will be provided in the morning.
Lunch will be provided at 11:00am for attendees who register.

Schedule:

Technical Talks
9:00 to 11:00 am
Teer 203

“Combined AFM and Vertical Light-Sheet Sideways Microscopy for Living Cell Studies”
Presenter: Dr. Mike Falvo

“New Modes for New Research – From Nanomechanics to Conductivity in Liquid”
Presenter: Dr. Thomas Mueller

Dimension Icon Demos
12:00 to 3:00 pm
Fitzpatrick 1557

Click here for more information, or contact Dr. Mark Walters (mark.walters@duke.edu), Director of SMIF. Register for the workshop here!

Technical Talk Abstracts:

“New AFM modes from Nanomechanics to Conductivity in liquid”
Dr. Thomas Meuller, Director of Applications Development

The Atomic Force Microscope has evolved from a laboratory sensation in the late 80’s to a widely used tool in materials and life science research today. Numerous innovations have driven this evolution, in particular the introduction of new imaging modalities and the parallel development of AFM probe technology, leading to a vast expansion of samples accessible and information revealed by AFM.

In this seminar we will focus on the very advances in Bruker’s AFM technology, including both, novel modes and probes, and the new information they make available enabling new research today. In life sciences, we will show how the evolution of modes from contact to Tapping and PeakForce Tapping has enabled a range of studies from sub molecular structure, where Pyne et al have studied such subtle phenomena as the variability of the DNA double helix structure, to imaging mammalian cells, with first and only AFM images of microvilli on live cells, and extending to cell biomechanical studies that unravel Nanomechanical signatures of cell state, disease progress, and the role of cell membrane receptors. In materials research, we will cover the latest advances in quantitative Nanomechanics of heterogeneous polymer samples and composites at high spatial resolution and at interfaces with PeakForce QNM, FastForce Volume, and contact resonance. Finally, we will cover our most recent extensions of AFM electrical measurements. Having pioneered conductivity measurements on soft and fragile samples with PeakForce TUNA, we have introduced analogous subsurface and impedance measurements with PeakForce sMiM, as well as conductivity in liquid and nanoscale electrochemical measurements with PeakForce SECM.

“Combined AFM and Vertical Light-Sheet Sideways Microscopy for Living Cell Studies”
Mike Falvo, Kellie Beicker, Evan Nelsen, Tim O’Brien, Richard Superfine

The ability of living cells to withstand external mechanical forces and convert mechanical stimuli into biological responses is essential to the physical integrity and biological functions of the cell. Abnormal cellular response to mechanical stimuli has been correlated with disease states, including cancer. Structural information related to rearrangement of the cytoskeletal structure, induced strains, and biochemical distributions are metrics for understanding cell response. However, structural information during applied stress is limited by our ability to image the cells under load, especially in the direction of applied force. In order to study the mechanics of single cells and subcellular components under load, we developed a unique imaging system that combines an atomic force microscope (AFM) with a new imaging technique called vertical light-sheet sideways microscopy (VLSSM). VLSSM combines pathway rotated imaging to facilitate the viewing of downward cell deformation by the applied force of the AFM tip and vertical light-sheet illumination to improve the signal-to-noise ratio achieved by illumination of a thin vertical slice of the cell. The combined AFM-VLSSM system enables simultaneous millisecond imaging and pico-Newton resolution force measurements. We use this new technique to observe force rupture events correlated with detachment of single membrane tethers from a functionalized AFM tip. Force rupture events are correlated with motion of the membrane and intracellular motion, including nuclear deformation attributed to nano-Newton forces applied to attachments at the cell membrane.